Tropic Marin NO2/NO3-Test

Determining nitrite/nitrate values

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Description

Tropic Marin's NO2/NO3 Test can be used to determine the nitrite/nitrate levels in saltwater and freshwater aquariums. All the necessary test cuvettes, reagents, dosing syringes and colour charts are included in the kit. The reagents can be bought in the refill pack after they have been used up.

The nitrogen cycle, nitrite and nitrate

The nitrogen cycle is one of the most important processes in an aquarium in which useful bacteria break down waste such as dead plants or fish excreta, mineralise them and render them harmless. This takes place during the nitrification process where bacteria convert ammonia and ammonium into nitrite which is toxic to aquarium inhabitants. The nitrite is then converted into non-toxic nitrate and then closes the nitrogen cycle. Plants absorb the nitrate and the cycle starts all over again.

Nitrite values must be kept low in all aquariums - the optimal value is below 0.2 mg/L. While the nitrate level in freshwater aquariums should be less than 50 mg/L, a value above 20 mg/L is not recommended in saltwater aquariums. In aquariums with corals, the nitrate value should be below 10 mg/L.

Instructions for use:

Since nitrite concentrations could influence the nitrate measurement, both must be measured before the nitrate measurement is carried out and nitrate values must be corrected if necessary.

Nitrite measurement:

  1. Shake the dropper bottle vigorously before use.
  2. Rinse the glass cuvette first with tap water and then with aquarium water.
  3. Fill the glass cuvette with 5 ml aquarium water with the dosing syringe.
  4. Add 5 drops of Reagent A to the glass cuvette, close with a stopper and shake the solution gently.
  5. Add 2 drops of Reagent C to the solution, seal with a stopper and shake gently.
  6. Let the solution sit for 3 minutes. Then place the opened glass cuvette on the white circles of the nitrite colour chart. Compare these with the colour fields located opposite. Look into the open cuvette from above. Move the sample on the colour chart until the colour of the cuvette and the colour field match. If the colours do not match, use an intermediate value.
  7. Read the measured nitrite value from the colour field.
  8. After measuring the nitrite value, rinse the glass cuvette and the syringe thoroughly with tap water.

Nitrate measurement:

  1. Shake the dropper bottle vigorously before use.
  2. Rinse the glass cuvette first with tap water and then with aquarium water.
  3. Fill the glass cuvette with 5 ml aquarium water with the dosing syringe.
  4. Add 10 drops of Reagent A to the glass cuvette, close with a stopper and shake the solution gently.
  5. Shake the bottle containing Reagent B vigorously in the horizontal position for 30 seconds. Add 10 drops of Reagent B to the glass cuvette, close with a stopper and shake the solution. After shaking, set them aside.
  6. After 3 minutes, add 4 drops of Reagent C to the solution, close with a stopper and shake the solution gently.
  7. Let the solution sit for 3 minutes. Then place the opened glass cuvette on the white circles of the nitrate colour chart. Compare these with the colour fields located opposite. Look into the open cuvette from above. Move the sample on the colour chart until the cuvette and the colour field above match. If the colours do not match, use an intermediate value.
  8. Read the measured nitrate value from the colour field. If necessary, the measured value must be corrected.
  9. After measuring the nitrate value, thoroughly rinse the glass cuvette and the syringe with tap water.

Included:

  • 1 x 20ml Reagent A
  • 1 x 20ml Reagent B
  • 1 x 10ml Reagent C
  • 1 x 10ml glass cuvette
  • 1 x 5ml dosing syringe
  • 1 x colour chart
  • 1 x instruction manual

Our expert Oliver Knott recommends:

Nitrite and nitrate concentrations in aquariums should be checked regularly and adjusted as necessary! High nitrate concentrations can disrupt coral growth or lead to algal blooms. On the other hand, concentrations that are too low result in nitrate deficiency.

Brands (Manufacturers): Tropic Marin

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